Across the Ryukyu islands is the northern most limit of the distribution of the coral reef. There is concern about the impact on the ecosystem by environmental pollution accompanying economic development in China and East Asia. This project investigates the influence of agricultural chemicals and paint from the bottom of ships on the coral reef, and measures the amount of harmful chemical substances contained in wildlife substances collected with the cooperation of local NPOs. Based on that data, consider potential risks, wildlife protection, marine conservation, provide information to policy makers and researchers involved in chemical substance management, and promote comprehensive coral reef conservation measures.