Cambodia's natural resources have encountered serious damages from a long-lasting civil war. Following the end of the Civil War, since 1990, Cambodia and various countries in the Mekong River Basin are in the process of transforming from self-sufficient to an export-oriented agriculture model. However, illegal logging and mass use of chemical fertilizer and agrichemicals to cultivate new land has caused soil degradation and polluted the water environment, which also has an adverse effect on the rural ecosystem and living environment. The purpose of these activities is conservation and sustainable use of natural resources for Satoyama regeneration in rural areas in the Centre, East and West of Cambodia. In order to achieve this, we will do the following: (1) Satoyama reproduction workshop of rural area, (2) actual condition survey of conservation of natural resources, (3) establish a tree planting and tree seedling nursing facility and the cultivate of tree planting, (4) Seminar on the use of natural resources, (5) Satoyama regeneration and afforestation, (6) Satoyama conservation leadership training, (7) Satoyama regeneration symposium, etc. By implementing afforestation in rural areas where natural resources are considerably impaired, we will regenerate ecologically rich natural resources and implement initiatives for local residents to conserve environment including soil and water and ultimately promote the sustainable use of natural resources in rural areas.